Current methods of administering busulfan to remove the endogenous germ cells cause hematopoietic toxicity, require special instruments and a narrow transplantation time.We use a direct testicular injection of busulfan method tokidoki hello kitty blind box for preparing recipients for SSC transplantation.Male ICR mice (recipients) were divided into four groups, and two experimental groups were treated with a bilateral testicular injection of 4 or 6 mg/kg/side busulfan (n = 60 per concentration group).Mice received an intraperitoneal injection (i.p.
) of 40 mg/kg busulfan (n = 60, positive control) and bilateral testicular injections of 50% DMSO (n = 60, negative control).Donor SSCs from RFP-transgenic C57BL/6J mice were introduced into the seminiferous tubules of each recipient testis via efferent duct injection kaiser copy stands on day 16-17 after busulfan treatment.Recipient mice mated with mature female ICR mice and the number of progeny was recorded.The index detected at day 14, 21, 28, 35 and 70 after busulfan treatment.Blood analysis shows that the toxicity of busulfan treated groups was much lower than i.
p.injection groups.Fertility was restored in mice treated with busulfan and donor-derived offspring were obtained after SSC transplantation.Our study indicated that intratesticular injection busulfan for the preparation of recipients in mice is safe and feasible.